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Finally, 200 L of 20 mM ammonium bicarbonate were added and the protein lysates were proteolytically cleaved overnight at 37C with trypsin (2.5 L of 0.1 g/l trypsin, Promega)

This increases cyclic AMP (cAMP) levels, initiating signalling pathways that relax smooth muscle fibres

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Cancer Res 64 : 236242 Girnita L, Girnita A, Brodin B, Xie Y, Nilsson G, Dricu A, Lundeberg J, Wejde J, Bartolazzi A, Wiman KG, Larsson O (2000) Increased expression of insulin-like growth factor I receptor in malignant cells expressing aberrant p53: functional impact

The symptoms are stimulated by the release of toxic bacterial components into the bloodstream when the bacteria break apart 3 4 5